1. a) Introduction to the science of microbiology. Major divisions of microbial world and relationship among them.
b) Different methods of classification of microbes and study of Bacteri, Fungi, Virus, Rickettsiae, Sprochetes.
2. a) Nutritional requirements, growth and cultivation of bacteria and virus, Study of different important media required for the growth of aerobic and anerobic bacteria and fungi. Differential media, enriched media and selective media, maintenance of lab cultures.
b) Different methods used in isolation and identification of bacteria with emphasis to different staining techniques and biochemical reactions. Counting of bacteria- Total and Viable counting techniques
3. a) Detailed study of different methods of sterilization including their merits and demerits. Sterilization methods for all pharmaceutical products. Detailed study of sterility testing of different pharmaceutical preparations.
Brief informaiton on validation
b) Disinfectants- Study of disinfectants, antiseptics, fungicidal and virucidal agents. Factors affecting their activation and mechanism of aciton. Evaluation of bactericial, bacteristatic, virucidal activities, evaluation of preservatives in pharmaceutical preparations
4 a) Immunology- Immunity, Definition, Classification, General Principles of natural immunity, Phagocytosis, acquired immunity (active and passive)
Antigens, Chemical nature of antigens structure and formation of antibodies, Antigen-Antibody reactions. Bacterial exotoxins and Endotoxins. Significance of toxoids in active immunity, Immunization programme and importance of booster dose.
b) Diagnostic tests: Schick's Test, ELISA test, Western Blot test, Southern Blot, PCR, Widal, QBC, Mantaux, Peripheral Smear. Study of Malarial parasite.
5 a) Microbial culture sensitivy testing: Interpretation of results. Principles and methods of different microbial assays, microbiological assay of pencillin, Streptomycin and Vitamin B2 and B12. Standardisaiton of vaccines and sera.
b) Study of infectious diseases: Typhoid, Tuberculosis, Malaria, Cholera, Hepatitis, Meningitis, Syphilis & Gonorrhea and HIV.