4. Spectroscopy:
Theoretical aspects, instumentaiton, elements of interpretation of data/spectra and application of analytical techniques be discussed on:
a. Absorption Spectroscopy:
-Theory of electronic, atomic and molecular spectra. Fundamental laws of photometry, Beer-Lambert's law, applicaiton and its deviation, limitation of Beer law, application of the law to single and multiple component analysis, measurement of equilibrium constant and rate constant by spectroscopy. Spectra of isolated chromophores, auxochromes, batho-chromic shift, hypsochromic shift, hyperchromic and hypochromic effect, effect of solvent on absorption spectra, molecular structure and infrared spectra.
-Instrumentation- Photometer, U.V.Visible spectrophotometer- sources of U.V.-Visible radiations, collimating systems, monochromators, samples cels and following detectors- Photocell, Barrier layer cell, Phototube, Diode array, applications of U.V.-Visible spectroscopy in pharmacy and spectrophotometric titrations.
-Infrared Spectroscopy: Vibrational transitions, frequency-structure correlaitons, Infrared absorption bands, Instrumentation-IR spectro-meter- sources of IR, Collimating systems, monochromators, sample cells, sample handling in IR spectroscopy and detectors- Thermocouple, Golay Cells, Thermistor, Bolometer, Pyroelectric detector, Applications of IR in pharmacy.
-Fluorimetric Analysis: Theory, luminescence, factors affecting fluorescence, quenching. Instrumentaiton, Applications, fluorescentindicators, study of pharmaceutically important compounds estimated by fluorimetry.
b.Flame Photometry: Theory, nebulisation, flame and flame temperature, interferences, flame spectrometric techniques and instrumentaiton and pharmaceutical applicaitons.
c. Atomic Absorption Spectrometry: Introduction, Theory, types of electrodes, instrumentation and applications.
d. Atomic Emission Spectroscopy: Spectroscopic sources, atomic emission spectrometers, photographic and photoelectric detection.
5. a. NMR and ESR (introduction only): Introduction, theroretical aspects and applicaitons.
b. Mass Spectroscopy: (Introduction only)- Fragmentation, types of ions produced mass spectrum and applicaitons.
c. Polarimetry: (Introduction only)- Introduction to optical rotatory dispersion, circular dichronism, polarimeter.
d. X-RAY Diffusion: (Introduciton only)- Theory, reciprocal lattice concept, diffration patterns and applicaitons.
e. Thermal Analysis: Introduciton, instrumentation, applications, and DSC and DTA.